A lightning separates the Magic School Bus into three pieces, scattering the class across the globe with different versions of Ms. Frizzle aboard each bus.
Ms. Frizzle's cousin, a pop star, performs worldwide on New Year's Eve and the class has been invited backstage. When the Bus undergoes a software update during the tour, it starts to glitch and malfunction.
Angela Franklin, one of Keesha's mothers, is a world-class cyclist. When she is framed for stealing the gold medal to this year's World Wheel Biking Games, the Frizz and the class play secret agent to uncover the thief's true identity.