Aerosmith is featured twice in the countdown: Run DMC and Aerosmith: Walk This Way (1986) and Aerosmith: Dude (Looks Like a Lady) (1987).
First original Top 40 of MTV 80s, since that previously it was released a "Top 40" version of the MTV 80s - Top 50 80s Classics That Made MTV! (2020).
The Top 40 hasn't host to introduce the music videos, and it hasn't opening or ending credits. It only broadcasts the music videos in countdown without pause or commercials but a brief channel ident promo each 20-30 minutes. In addition, it shows titles (solo singer/music band, song name and album name) at the start and ending of each one, and its number in the countdown.
Of the 40 music videos featured in the countdown, there is none in black and white. Europe: The Final Countdown (1986) is the only one to alternate color with black and white. The rest of the music videos are in color.
In the countdown there are featured two duets: David Bowie & Mick Jagger: Dancing in the Street (1985) and Run DMC and Aerosmith: Walk This Way (1986).