"The Hundredth Smurf" is based on the 1962 comic story titled "Le Centième Schtroumpf" (The Hundredth Smurf) which appeared as a detachable supplement in Spirou (the last one). There are major differences from the cartoon adaption:
- Smurfette is absent in the original comic story as it predates her 1966 debut in "La Schtroumpfette" ("The Smurfette" in English).
- The dance involving one hundred Smurfs is not part of a ritual to prevent their village from facing a hundred years of bad luck, but to celebrate a festival which is held every 654 years.
- Vanity's reflection ("The Hundredth Smurf") became a regular Smurf by passing through the mirror he originated, not when he and the mirror were again struck by lightning as depicted in the cartoon version.
Vanity's reflection (The Hundreth Smurf) is the first Smurf who was born un-naturally, but unlike Smurfette and latter on Sassette, he was not created by magic but by lightning hitting Vanity's mirror.
The source of "The Hundred Smurf" (the 1962 comic story titled "Le Centième Schtroumpf") is the first time that most Smurfs other than Papa Smurf were given names (Jokey is the exception, as his name is called out whenever he plays a prank as was the case in the sources of "The Purple Smurfs" and "Haunted Smurfs"). In previous comic stories before it, while some Smurfs had distinguished personalities (e.g. Brainy), they were usually un-named.