A view inside the car during the chase shows lighting on Michael's face that does not vary, even though he is supposed to be driving down a street, where the light from street-lamps and other sources would be constantly changing.
When escaping from their pursuers in Turin, the pair run past the same illuminated signs twice.
When escaping in a car at night, a long shot from overhead shows the car swinging round on a crossroad with no other cars in sight, yet the view inside the car looking through the back window shows another car following close behind.
When running for the train, Giulia is not wearing the jacket she had the previous evening, and is not carrying any luggage.
The car chase sequence set in "London" (but filmed in California) features numerous flipped/reversed frames in order to create the impression that cars are indeed driving on the correct British side of the road. However in doing this, countless street signs and number plates also appear reversed.
During the foot chase in "Turin", numerous English-language signs are visible. Additionally, Michael and Giulia pass the same block of cafés twice.
The book of Chronicles is mentioned quoting the "Christ Jesus". Both Chronicles I and Chronicles II are old testament books and have no reference to the "Christ Jesus".
When Michael gets into the car after the robbery, a crew member switches on a light to illuminate him from outside the car, and it is plainly visible, reflected in the windscreen.
Da Vinci apparently gives the longitude and latitude of the hidden treasure in Afghanistan, where Giulia Pedina is able to guide Michael Archer using modern GPS. The issue of how to work out longitude wasn't settled until about 230 years after Da Vinci died, and the Greenwich Meridian from which coordinates are based not agreed until 1884, some 370 years after the death of Da Vinci.