Peter Venkman jokingly mentions Howard the Duck running for president (something he did in his own comic series).
The voice actors were recorded on August 4, 1986.
During the episode there are three people that resemble "The Three Stooges." Once after slimer has multiplied into small slimers, and again after slimer has become a large and blue. Both times they get slimmed.
This episode suggests that the Mayor was in an election year indicating the year to be 1985 since there was no other mayoral election until 1989 in New York City.
A King Kong joke was made by Peter twice during the climax of the episode. Scenes of a giant Slimer grabbing Janine through a window, climbing the empire state building, and fighting aircraft were all reminiscent of the King Kong Story.