When they take the General Lee for it's first drive you can see the back window being fine, then during the drive there's a shot of it being broken out, but when they arrive back it's whole again.
The stains Daisy makes on the bartenders T-shirt (when she applies for a job) disappear in the next shot.
When Luke and Bo were running away from Boss Hogg's mansion, Luke was wearing a open shirt without white t-shirt under it. You can see his bare chest. When their car was almost hit by a bulldozer, Luke was only wearing a white t-shirt.
When Uncle Jesse is talking to Luke and Bo in the garage, his braid appears in one shot where he has his arms over the shoulders of Luke and Bo. The braid is not there in all other shots of that scene.
The car that Bo finds in the water is clearly not a Dodge Charger. When he's by the door you can clearly see the door has a window frame. Dodge Chargers have frameless windows.
When the General is pulled out of the water the flag is already painted on top, However in next scene when Luke is setting on window there is no flag on the General.
This movie is supposed to take place before the 2005 film. In the beginning of the 2005 film, General Lee had no confederate flag, and the 01 was sloppily painted on there. It was made to look like the original later on in the film. In this film, it says it always looked like it originally did.
Near the end of the movie, Bo says, "Hopefully she'll last long enough to do what we gotta do" while referring to the General Lee. The General is always referred to as a "he", never a "she".
In the original series, Bo and Luke's parents supposedly died in a tragic car accident, along with Daisy's. In the film, Jesse makes many references to the boys' parents being alive.
In the original series, Luke was the oldest, followed by Daisy, then Bo. In this film, Daisy is the oldest, and Luke is the middle.