While climbing the Channel 6 tower to April's office, Donatello verbally wishes for the "Anti-gravity boots from episode six". This is breaking the fourth wall and the first acknowledgement by the turtles that they are part of a TV show. Also, he makes an error when mentioning the "Anti-gravity boots from episode 6" since it was actually episode 23 (the 5th episode of Season 3), Sky Turtles (1989), that he is referencing as the anti-gravity boots were a main part of that episode.
Erma pines after Errol Leslie Flynn, an Australian-born actor who achieved fame in 1930's Hollywood due to his roles as a romantic swashbuckler.
Michelangelo makes an error when he mentions the "gravity boots from episode 6". It was actually episode 5: Sky Turtles, that he is referencing.
The Shredder and his goons do not appear in this episode.
The Rat King's first appearance in the series. He's also the main antagonist in this episode.