The Queen Bee's ultimatum was signed "The Nasty Bee Coalition". This show aired on the NBC network.
Frank N. Stein has, protruding from his forehead, threaded bolts with wing-nuts screwed on. In the 1931 Boris Karloff movie the monster had heavy gauge electrodes in his neck to receive the life giving lightning-bolt electricity. Decades later these somtimes became machine bolts and migrated to the head. Frank's wing-nuts are almost certainly a make up artist's joke about that.