Tricia Takanawa does a new report on the dangers of having sex with strangers in motel rooms, in which she actually has sex with a stranger (Quagmire) on camera in a motel room. This is a reference to a skit from "The Groove Tube" where a journalist does a news report on the dangers of soliciting prostitution in the ghetto, where he actually has sex with a hooker on camera in a motel room.
Pre-title scene recreates the beginning of an episode of this show, then Stewie appears and reveals that he has wrecked the Land of Make-Believe, then he zaps Mr. Rogers with a ray gun.