Despite allowing the use of the Green Lantern Corps and Sinestro in another episode, Black Manta and Aquaman do not appear in this Legion of Duck Doom episode but rather Black Eel and Seaman. Sinestro, one could note, did serve as a member of the Legion of Doom during Challenge of the Super Friends.
In "Til Doom Do Us Part," Agent Roboto assembles some of Duck Dodgers' old enemies to form the Legion of Duck Doom (a parody of DC Comics' the Legion of Doom and the Superman Revenge Squad). It is made up of Crusher, Fudd, a Catapoid, Count Muerte, New Cadet, Black Eel, Nasty Canasta, Commandante Hilgalo, Baby-Faced Moonbeam, Long John Silver the 23rd, K'Chutha Sa'am, and the Tasmanian Warrior.