Having King Tut's female accomplice named Nefertiti implies a romantic connection between the historical Tutankhamun and Nefertiti. The real Nefertiti, was a queen to Tutankhamun's father, the pharaoh Akhenaten (she was not, however, Tutankhamun's mother - that was another wife of Akhenaten, who was also his sister).
In this episode (1.27) Tut addresses the people of Gotham as "Subjects and Helots." Unfortunately for the episodes writer, they didn't do any research. Helot's were the slave class of Laconia, also known as Sparta, which is in Greece, not Egypt.
When Batman is investigating the sphinx in the park, he identifies a costume as "...belonging to the Fourteenth Dynasty, King Tut's Dynasty". Later, in the museum, Bruce Wayne identifies the sarcophagus as also being from the Fourteenth Dynasty, and dates it to about "1500 BC". He was wrong on both counts. Tutankhamun's reign ran from approximately 1333 BC to 1323 BC, and he was a member of the Eighteenth Dynasty, not the Fourteenth.