Hampton happens to have vomited on Plucky's comic book (though the perspective was only shown outside the car since it is considered repulsive to show characters vomiting, and this was only heard in dialogue); but in a later scene, Plucky seems to be holding that same comic book, and it's perfectly intact with no damage on it whatsoever.
In the final scene when everyone returns to Acme Looniversity, Calamity, Mary, Furrball, Li'l Sneezer, and Vinnie were wearing their skating clothes during the lyrics "We've been outdone!"; yet in the next few seconds, the skating clothes and Vinnie are gone and Mary is back to her usual clothes.
In the scene where Wade backs up the car to pick up Plucky on the road when Plucky wanted to tag with them badly, the gear shift lever appeared to be mounted between the driver and passenger seats; but in every other shot, the gear shift is mounted on the steering column and the whole front seat is a bench seat, which the car seats up to 6 occupants at the most.
The license plate holder on the rear of Elmyra's family's station wagon up to the wild animals carjacking it in retaliation changes positions from the hatch to the bumper and vice-versa between shots.
Elmyra's family's station wagon's electric windows have slanted edges in exterior shots, but are perfectly rectangular in interior shots.
In Fifi la Fume's line "Whatever your location" during the titular musical number, she points to posters and signs of what should be countries around the world. Boise is shown on the list of destinations. Boise is not a country, but the capital of the U.S. state of Idaho.
Fifi la Fume sneaks into the Acme Resort Hotel so she can get Johnny Pew's photograph and meet him to have it signed. In reality, this kind of behavior is called "ghosting" - which is a crime, and can result in removal from the premises at the person's own expense.
Hamton J. Pig and his family with Plucky Duck tagging along spend their entire summer vacation on a road trip to Happy World Land and back to Acme Acres just shortly before school goes back in session. Most families would never spend an entire season away from home, especially considering the fact that vacations are costly and if anything goes wrong with an automobile that's being driven - driving to the costs of routine maintenance.
During the movie's titular musical number that is the vacation version of the series' main theme, just right before the title card, the seven characters shown: Buster, Babs, Plucky, Hamton, Furrball, Dizzy Devil, and Elmyra are all in their summer attire, but everyone except for Furrball wear shades.
During the movie's opening musical number "Waiting for the Clock", when Hamton pulls down a map of the United States, the states depicted are shown to be poorly illustrated/referenced with some states appearing smaller/thinner than they do in reality and if Oklahoma were divided into two states.
Buster and Babs Bunny flood Acme Acres, but none of the buildings being submerged result in any damage whatsoever.
During the opening credits, in the classroom, Shirley's hands are relaxed while her pencil appears to be writing on its own.
Elmyra's family's car has no seat belts, but she sits in a booster seat due to her small stature. It would not be able to stay in place without any support from seat belts intended to prevent it from overturning from the effects of the car's movements.
The USA DVD cover depicted Babs, Buster, Dizzy Devil, Elmyra, Hamton, and Plucky riding on one of the rides on Happy World Land; yet in the actual movie, only Plucky along with Hamton, Wade, Winnie, and Uncle Stinky went to Happy World Land while all of the other characters took different adventures.
The USA DVD incorrectly states that it only includes English and Spanish audio and subtitles, but it also has Portuguese audio and subtitles as well.
During the song at the end of the movie, Calamity Coyote is shown singing along with everyone else. He is supposed to be a mute.
Buster's gloves disappear at one point in the "Dueling Banjos" parody.
In the titular music number, Fifi la Fume is shown considering going to Paris due to her French nature with using random French words as she points to a poster of Paris that would hint her planned vacation. Instead, during the plot of the movie, she meets movie star Johnny Pew despite what her line in the titular musical number suggests.