Has a tattoo on the side of her finger that reads "12 21:, which stands for Romans, Chapter 12, Verse 21: "Never be overcome by evil, but
overcome evil with good.".
Suffers from a severe phobia of magicians and conventional ovens. (Microwaves don't bother her.).
On July 7, 2020, she announced her retirement from acting. She initially planned to do so after leaving NCIS (2003), but changed her mind after accepting the lead role in the short-lived comedy series Broke (2020).
She was the lead singer in the all-girl punk band, Lo-Ball.
Co-owner, with Matthew Sandusky and Darren Greenblatt, of Donna Bell's Bake Shop in New York City. The shop is named for Pauley's mother.
For much of her twenties, sported a bald head that she shaved with a razor.