- Prologue (Little Shop of Horrors)Written by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob Gaudio
- The Gospel Truth (Prologue)Music by Alan MenkenLyrics by David Zippel
- I Can't Help Myself (Sugar Pie Honey Bunch)Performed by Levi Stubbs, Abdul 'Duke' Fakir, Renaldo 'Obie' Benson, Lawrence Payton and The Four Tops
- Baby Got BackWritten by Sir Mix A LotPerformed by Eddie Murphy
- Do You Really Want to Hurt Me?Performed by Conrad Vernon
- Staying AlivePerformed by John Lithgow
- Grow For MeWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob Gaudio
- Suddenly, SeymourWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob Gaudio
- Da-DooWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob GaudioPerformed by Rick Moranis, Michelle Weeks, Tichina Arnold, Tisha Campbell and The Do Wops (Nick Curtis, Peter Beckett, Alan Carvell, Phil Nicholl, Lindsay Benson and Donny Gerrard)
- Somewhere That's GreenWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob GaudioPerformed by Ellen Greene
- Sal Tlay Ka SitiPerformed by Nikki M. James
- Mushnik and SonWritten by Howard Ashman and Alan MenkenPerformed by Rob Bartlett
- Dentist!Written by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob GaudioPerformed by Steve Martin, Michelle Weeks, Tichina Arnold and Tisha Campbell with Robby Merkin and Robert Billig
- House of the Rising SunPerformed by Bill Murray
- Dr. ZaiusWritten by Alf Clausen and Jack Barth
- Skid Row (Downtown)Written by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob Gaudio
- Tossed Salads and Scrambled EggsMusic by Bruce MillerLyrics by Darryl PhinnesseePerformed by Kelsey Grammer
- Da-Doo (Little Shop of Horrors Recapmix)Produced by Sol HarrisPerformed by Sol Harris
- Don't Feed The PlantsWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob Gaudio
- Mean Green Mother from Outer SpaceWritten by Howard Ashman and Alan MenkenArranged and Adapted by Bob Gaudio and Robby MerkinProduced by Bob GaudioPerformed by Levi Stubbs and Chorus (Oren Waters, Maxine Waters Willard, Julia Waters, Lynn Davis, Tommy Funderburk, Siedah Garrett, Debra Dobkin, Luther Waters, Jay Gruska, Joe Pizzulo, Donny Gerrard, Monalisa Young and Gene Morford)
- Butter Outta CreamMusic by Marc ShaimanLyrics by Marc Shaiman and Scott WittmanPerformed by Aaron Tveit and Tom Wopat
- Prepare for Trouble
- Bend ItMusic by Howard GoodallLyrics by Charles HartPerformed by Natalie Dew and Jamie Campbell Bower
- And Eve Was WeakMusic by Michael GoreLyrics by Dean PitchfordPerformed by Maureen McGovern and Annie Golden
- ChristmastownMusic by Matthew SklarLyrics by Chad BeguelinPerformed by Sebastian Arcelus
- Keep on StandingMusic by Stephen FlahertyLyrics by Lynn AhrensPerformed by Drew Sarich
- Just One DayMusic by Tom KittLyrics by Brian YorkeyPerformed by Emma Hunton and Heidi Blickenstaff
- SolidarityMusic by Elton JohnLyrics by Lee HallPerformed by the cast of Billy Elliot
- I Know It's TodayMusic by Jeanine TesoriLyrics by David Lindsay-AbairePerformed by Sutton Foster
- Opening NightWritten by Mel BrooksPerformed by the cast of The Producers
- Omigod You GuysWritten by Nell Benjamin and Laurence O'KeefePerformed by Laura Bell Bundy and the cast of Legally Blonde
- If I Had My Time AgainWritten by Tim MinchinPerformed by Barrett Doss and Andy Karl
- The Locker Room OrgyPerformed by Susan Schwartz
- Meet the PlasticsMusic by Jeff RichmondLyrics by Nell Benjamin
- Live by the HookWritten by Gary Barlow and Eliot KennedyPerformed by Kelsey Grammer
- Cabin in the WoodsLyrics by George Reinblatt
- What the Fuck Was That?Lyrics by George ReinblattPerformed by Garrett Summers and Chris Holloway
- James Bond ThemeMusic By Monty Norman
- American Beauty ThemeMusic By Thomas Newman
- A Man for All SeasonsWritten by Hans Zimmer and Robbie WilliamsPerformed by Robbie Williams
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